Safety by Design: Pre-IND Risk Considerations for In Vivo Gene Editing Programs
Genotoxicity, immunogenicity, off-target editing — a framework for building safety considerations into the LNP formulation screening process before animal studies begin.
Resources · Blog
LNP & gene editing research blog
Technical writing from the Gendelivr research team on formulation science, delivery mechanics, and computational screening. 12 articles.
Delivering Base Editors: Why Cargo Size Changes Everything
Base editors and prime editors are larger than standard SpCas9 constructs. How larger cargo size reshapes the LNP formulation optimization problem.
Endosomal Escape: The Rate-Limiting Step in LNP-Mediated Gene Delivery
Only ~1-2% of endocytosed LNPs successfully escape to the cytoplasm. An examination of proton sponge effect, membrane fusion models, and formulation levers.
mRNA Stability Inside LNPs: Formulation Parameters That Matter
RNA integrity starts degrading before the LNP reaches its target. How ionizable lipid pKa, internal pH, and aqueous core composition affect cargo stability.
Engineering Tropism in AAV Capsids: Surface Loop Mutations and Their Consequences
Natural AAV serotypes miss the target cells for many CNS and hepatic programs. A technical review of rational capsid engineering approaches for re-directing tropism.
How Computational Screening Changes the Economics of LNP Discovery
Running 10,000 virtual formulations costs a fraction of bench synthesis. A breakdown of the computational cost structure versus wet lab equivalent.
AAV vs. LNP for In Vivo Gene Therapy: A 2025 Decision Framework
Payload size, immunogenicity, redosing, manufacturing cost — a structured comparison to help gene therapy programs choose the right delivery modality.
PEG-Lipid Density and Immunogenicity: Navigating the Tradeoff
Higher PEG density reduces protein corona formation but suppresses cellular uptake. How to model the PEG tradeoff computationally before running a single mouse study.
ApoE-Mediated Hepatic Uptake: What It Means for LNP Formulation Strategy
Apolipoprotein E adsorption onto LNP surfaces is the dominant driver of liver-targeting efficiency — here's how formulation parameters modulate it.
Anatomy of an 18-Month LNP Development Timeline — And Where to Cut It
A phase-by-phase dissection of the bench-to-IND timeline for LNP delivery programs, identifying where computational screening creates the most leverage.
The Three Barriers Blocking In Vivo CRISPR Delivery
Serum protein adsorption, endosomal entrapment, and immune clearance — a technical breakdown of the delivery challenges every gene editing program faces.
Ionizable Lipid Selection: The Bottleneck in LNP Formulation Optimization
Why ionizable lipid chemistry dominates transfection efficiency, and how computational pre-screening narrows the design space from thousands to dozens of candidates.